25-500 bp DNA Marker abx461022|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司

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產品名稱：25-500 bp DNA Marker
產品型號：abx461022
產品廠商：abbexa
產品文檔：

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簡單介紹：

25-500 bp DNA Marker

詳情介紹：

25-500 bp DNA Marker contains 12 DNA fragments ranging from 25 to 500 base pairs. The 100 bp and 200 bp bands have the highest intensity for easy identification. Ready-to-use, the blue dye in the loading buffer runs at approximately 4 bp on a 1% gel. Can optionally be used for mass determination. 1 test is equivalent to 1 lane.

Target

25-500 bp DNA Marker

Tested Applications

SDS-PAGE

Recommended dilutions

Recommended Agarose % (w/v)	Effective Separation Range of Linear DNA (bp)
0.3	5000 - 60,000
0.5	1000 - 20,000
0.7	800 - 12,000
1.0	500 - 10,000
1.2	400 - 7000
1.5	200 - 3000
2.0	100 - 2000
2.5	50 - 100
3.0	10 - 75

Note: Circularized plasmids can be cut and linearized by an appropriate restriction enzyme to measure their molecular weight.

Form

Liquid

Storage

Store all components at -20 °C. Avoid repeated freeze/thaw cycles.

Validity

Up to 12 months.

Buffer

Contains a restriction digest plus one or more PCR products.

Availability

Shipped within 3-7 working days.

Note

This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.

Directions for use

Thaw, mix, and briefly centrifuge before use. Vortex the tube, then add 5 µl of ladder per lane. There is no need to add loading buffer to the ladder. The provided 5X Sample Loading Buffer can be used for sample loading; it should not be used for diluting the ladder.

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產品名稱：25-500 bp DNA Marker