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Alpha-Methylacyl-CoA Racemase (AMACR) Antibody abx227135|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司
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  • 產品名稱：Alpha-Methylacyl-CoA Racemase (AMACR) Antibody

  • 產品型號：abx227135
  • 產品廠商：abbexa
  • 產品文檔：
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簡單介紹：
Alpha-Methylacyl-CoA Racemase (AMACR) Antibody
詳情介紹：
Alpha-Methylacyl-CoA Racemase (AMACR) Antibody is a Rabbit Monoclonal antibody for the detection of P504S (AMACR).

Target Alpha-Methylacyl-CoA Racemase (AMACR)
Clonality Monoclonal
Clone P495
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the N-terminal region of human P504S.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC Q9UHK6 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  • Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  • Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  • Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  • Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  • Rinse twice in distilled water, 5 minutes each.
  • Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  • Wash twice in distilled water, 5 minutes each.
  • Antigen retrievals: immerse the slide in Citrate buffer, pH 6.0, and incubate in a water bath for 25 minutes at 95-97 °C.
  • Remove the slide from the water bath and allow to stand at room temperature (in Citrate buffer, pH 6.0) for 15 minutes.
  • Rinse twice in distilled water, 5 minutes each.
  • Wash twice in Wash Buffer, 5 minutes each.
  • Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  • Wash 3 times with Wash Buffer, 5 minutes each.
  • Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  • Wash 3 times in Wash Buffer, 5 minutes each.
  • Apply the DAB chromagen for 1-3 minutes.
  • Wash twice in water, 5 minutes each.
  • Stain in hematoxylin for 5 minutes.
  • Wash 3 times in water, 2 minutes each.
  • Mount the slide for observation.
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