产品详情

  • 产品名称：Human APLN (Apelin) Competitive ELISA (STJE0002239)

  • 产品型号：STJE0002239
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human APLN (Apelin) Competitive ELISA (STJE0002239)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This APLN Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: APLN
Gene ID: 8862
Uniprot ID: APEL_HUMAN
Specificity: This kit recognizes Human APLN in samples. No significant cross-reactivity or interference between Human APLN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Several active peptides may be produced by proteolytic processing of the peptide precursor.
Function Endogenous ligand for the apelin receptor (APLNR). Drives internalization of the apelin receptor. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR. Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking.the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation. Mediates myocardial contractility in an ERK1/2-dependent manner. May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives.
Protein Name Apelin
Apj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Secreted
Extracellular Space
Abundantly Secreted In The Colostrum
Lower Level In Milk
Decreases Rapidly Within Several Days After Parturition In Milk
But Is Still Detectable Even In Commercial Milk
Alternative ELISA Names Apelin ELISA kit
Apj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13 ELISA kit
APLN ELISA kit
APEL ELISA kit
Specificity This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 90
EDTA plasma(n=8) 91-102 96
Cell culture media(n=8) 89-100 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 197.40 537.10 1359.90 204.30 499.70 1350.70
Standard deviation 11.10 31.70 50.30 11.00 26.00 56.70
CV (%) 5.62 5.90 3.70 5.38 5.20 4.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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