产品详情

  • 产品名称：Human ATXN2 (Ataxin-2) Sandwich ELISA (STJE0002288)

  • 产品型号：STJE0002288
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ATXN2 (Ataxin-2) Sandwich ELISA (STJE0002288)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This ATXN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ATXN2
Gene ID: 6311
Uniprot ID: ATX2_HUMAN
Specificity: This kit recognizes Human ATXN2 in samples. No significant cross-reactivity or interference between Human ATXN2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.
Protein Name Ataxin-2
Spinocerebellar Ataxia Type 2 Protein
Trinucleotide Repeat-Containing Gene 13 Protein
Cellular Localisation Cytoplasm
Alternative ELISA Names Ataxin-2 ELISA kit
Spinocerebellar Ataxia Type 2 Protein ELISA kit
Trinucleotide Repeat-Containing Gene 13 Protein ELISA kit
ATXN2 ELISA kit
ATX2 ELISA kit
SCA2 ELISA kit
TNRC13 ELISA kit
Specificity This kit recognizes Human ATXN2 in samples. No significant cross-reactivity or interference between Human ATXN2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 100
EDTA plasma (n=8) 91-107 98
Cell culture media (n=8) 91-104 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.67 4.90 22.65 2.75 5.10 21.61
Standard deviation 0.15 0.25 0.83 0.15 0.29 0.67
CV (%) 5.62 5.10 3.66 5.45 5.69 3.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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