产品详情
简单介绍:
Human BAK1 (Bcl-2 homologous antagonist/killer) CLIA Kit (STJC0000311)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.09ng/mL |
Detection Limit: | 0.16~10ng/mL |
Short Description: | This human BAK1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | BAK1 |
Gene ID: | 578 |
Uniprot ID: | BAK_HUMAN |
Specificity: | This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Tissue Specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
Function | Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases. |
Protein Name | Bcl-2 Homologous Antagonist/Killer Apoptosis Regulator Bak Bcl-2-Like Protein 7 Bcl2-L-7 |
Database Links | Reactome: R-HSA-111452 Reactome: R-HSA-111457 Reactome: R-HSA-5620971 |
Cellular Localisation | Mitochondrion Outer Membrane Single-Pass Membrane Protein |
Alternative CLIA Names | Bcl-2 Homologous Antagonist/Killer CLIA kit Apoptosis Regulator Bak CLIA kit Bcl-2-Like Protein 7 CLIA kit Bcl2-L-7 CLIA kit BAK1 CLIA kit BAK CLIA kit BCL2L7 CLIA kit CDN1 CLIA kit |
Specificity | This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 101-115 | 107 |
EDTA plasma(n=8) | 85-96 | 90 |
Cell culture media(n=8) | 92-108 | 98 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.47 | 0.84 | 4.13 | 0.50 | 0.83 | 3.95 |
Standard deviation | 0.06 | 0.08 | 0.44 | 0.06 | 0.08 | 0.32 |
CV (%) | 12.77 | 9.52 | 10.65 | 12.00 | 9.64 | 8.10 |
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