产品详情

  • 产品名称：Human C5 (Complement C5) Sandwich ELISA (STJE0002497)

  • 产品型号：STJE0002497
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human C5 (Complement C5) Sandwich ELISA (STJE0002497)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This C5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: C5
Gene ID: 727
Uniprot ID: CO5_HUMAN
Specificity: This kit recognizes Human C5 in samples. No significant cross-reactivity or interference between Human C5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Complement C5
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5 Alpha' Chain
Database Links Reactome: R-HSA-166665
Reactome: R-HSA-174577
Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative ELISA Names Complement C5 ELISA kit
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5 Alpha' Chain ELISA kit
C5 ELISA kit
CPAMD4 ELISA kit
Specificity This kit recognizes Human C5 in samples. No significant cross-reactivity or interference between Human C5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-100 95
EDTA plasma (n=8) 95-108 100
Cell culture media (n=8) 93-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 253.77 643.02 2124.74 257.27 684.79 2226.57
Standard deviation 15.18 30.80 102.41 15.51 38.42 98.86
CV (%) 5.98 4.79 4.82 6.03 5.61 4.44
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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