产品详情
简单介绍:
Human APOC2 (Apolipoprotein C-II) Sandwich ELISA (STJE0002247)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 1.88ng/mL |
Detection Limit: | 3.13~200ng/mL |
Short Description: | This APOC2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | APOC2 |
Gene ID: | 344 |
Uniprot ID: | APOC2_HUMAN |
Specificity: | This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing. Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma. |
Function | Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL. |
Protein Name | Apolipoprotein C-Ii Apo-Cii Apoc-Ii Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii Proapoc-Ii |
Database Links | Reactome: R-HSA-8963888 Reactome: R-HSA-8963889 Reactome: R-HSA-8963901 Reactome: R-HSA-8964058 Reactome: R-HSA-9029569 Reactome: R-HSA-975634 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Apolipoprotein C-Ii ELISA kit Apo-Cii ELISA kit Apoc-Ii ELISA kit Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii ELISA kit Proapoc-Ii ELISA kit APOC2 ELISA kit APC2 ELISA kit |
Specificity | This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 85-97 | 90 |
EDTA plasma (n=8) | 90-102 | 97 |
Cell culture media (n=8) | 86-96 | 91 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 11.00 | 19.50 | 83.60 | 12.10 | 19.90 | 86.30 |
Standard deviation | 0.80 | 0.80 | 3.90 | 0.80 | 0.90 | 2.90 |
CV (%) | 7.27 | 4.10 | 4.67 | 6.61 | 4.52 | 3.36 |
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