产品详情
简单介绍：
Human COL17A1 (Collagen alpha-1 (XVII) chain) Sandwich ELISA (STJE0002481)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 1.88ng/mL |
| Detection Limit: | 3.13~200ng/mL |
| Short Description: | This COL17A1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | COL17A1 |
| Gene ID: | 1308 |
| Uniprot ID: | COHA1_HUMAN |
| Specificity: | This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | The intracellular/endo domain is disulfide-linked. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544. |
| Function | May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies. |
| Protein Name | Collagen Alpha-1(Xvii Chain 180 Kda Bullous Pemphigoid Antigen 2 Bullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen 120 Kda Linear Iga Dermatosis Antigen Linear Iga Disease Antigen 1 Lad-1 - 97 Kda Linear Iga Disease Antigen 97 Kda Linear Iga Bullous Dermatosis Antigen 97 Kda Lad Antigen 97-Lad Linear Iga Bullous Disease Antigen Of 97 Kda Labd97 |
| Database Links | Reactome: R-HSA-1442490 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-446107 Reactome: R-HSA-8948216 |
| Cellular Localisation | Cell Junction Hemidesmosome Membrane Single-Pass Type Ii Membrane Protein Localized Along The Plasma Membrane Of The Hemidesmosome 120 Kda Linear Iga Disease Antigen: Secreted Extracellular Space Extracellular Matrix Basement Membrane Exclusively Localized To Anchoring Filaments Localized To The Epidermal Side Of Split Skin 97 Kda Linear Iga Disease Antigen: Secreted Localized In The Lamina Lucida Beneath The Hemidesmosomes |
| Alternative ELISA Names | Collagen Alpha-1(Xvii Chain ELISA kit 180 Kda Bullous Pemphigoid Antigen 2 ELISA kit Bullous Pemphigoid Antigen 2 Cleaved Into - 120 Kda Linear Iga Disease Antigen ELISA kit 120 Kda Linear Iga Dermatosis Antigen ELISA kit Linear Iga Disease Antigen 1 ELISA kit Lad-1 - 97 Kda Linear Iga Disease Antigen ELISA kit 97 Kda Linear Iga Bullous Dermatosis Antigen ELISA kit 97 Kda Lad Antigen ELISA kit 97-Lad ELISA kit Linear Iga Bullous Disease Antigen Of 97 Kda ELISA kit Labd97 ELISA kit COL17A1 ELISA kit BP180 ELISA kit BPAG2 ELISA kit |
| Specificity | This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 94-110 | 101 |
| EDTA plasma (n=8) | 91-103 | 97 |
| Cell culture media (n=8) | 93-108 | 100 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 9.57 | 26.33 | 87.89 | 10.51 | 26.52 | 91.96 |
| Standard deviation | 0.65 | 1.43 | 3.70 | 0.73 | 1.47 | 3.87 |
| CV (%) | 6.79 | 5.43 | 4.21 | 6.95 | 5.54 | 4.21 |
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