产品详情

  • 产品名称：Human CXCL12 (Stromal cell-derived factor 1) CLIA Kit (STJC0000047)

  • 产品型号：STJC0000047
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CXCL12 (Stromal cell-derived factor 1) CLIA Kit (STJC0000047)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CXCL12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CXCL12
Gene ID: 6387
Uniprot ID: SDF1_HUMAN
Specificity: This kit recognizes Human SDF-1 in samples. No significant cross-reactivity or interference between Human SDF-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Post Translational Modifications Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Function Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development.required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells.
Protein Name Stromal Cell-Derived Factor 1
Sdf-1
Hsdf-1
C-X-C Motif Chemokine 12
Intercrine Reduced In Hepatomas
Irh
Hirh
Pre-B Cell Growth-Stimulating Factor
Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-376176
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-9018519
Cellular Localisation Secreted
Alternative CLIA Names Stromal Cell-Derived Factor 1 CLIA kit
Sdf-1 CLIA kit
Hsdf-1 CLIA kit
C-X-C Motif Chemokine 12 CLIA kit
Intercrine Reduced In Hepatomas CLIA kit
Irh CLIA kit
Hirh CLIA kit
Pre-B Cell Growth-Stimulating Factor CLIA kit
Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67 CLIA kit
CXCL12 CLIA kit
SDF1 CLIA kit
SDF1A CLIA kit
SDF1B CLIA kit
Specificity This kit recognizes Human SDF-1 in samples. No significant cross-reactivity or interference between Human SDF-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 102-116 108
EDTA plasma(n=8) 99-113 107
Cell culture media(n=8) 94-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.61 271.56 923.29 95.05 285.78 961.32
Standard deviation 8.51 23.30 61.68 11.08 24.23 95.75
CV (%) 8.90 8.58 6.68 11.66 8.48 9.96
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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