产品详情

  • 产品名称：Human COL7 (Collagen Type 7) Sandwich ELISA (STJE0002478)

  • 产品型号：STJE0002478
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human COL7 (Collagen Type 7) Sandwich ELISA (STJE0002478)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This COL7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: COL7A1
Gene ID: 1294
Uniprot ID: CO7A1_HUMAN
Specificity: This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Protein Name Collagen Alpha-1(Vii Chain
Long-Chain Collagen
Lc Collagen
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-204005
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-3000157
Reactome: R-HSA-5694530
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative ELISA Names Collagen Alpha-1(Vii Chain ELISA kit
Long-Chain Collagen ELISA kit
Lc Collagen ELISA kit
COL7A1 ELISA kit
Specificity This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 96
EDTA plasma (n=8) 97-110 102
Cell culture media (n=8) 87-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.20 4.30 0.50 1.30 4.50
Standard deviation 0.04 0.06 0.15 0.03 0.06 0.25
CV (%) 8.00 5.00 3.49 6.00 4.62 5.56
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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