产品详情

  • 产品名称：Human IL25 (Interleukin-25) Sandwich ELISA (STJE0003130)

  • 产品型号：STJE0003130
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL25 (Interleukin-25) Sandwich ELISA (STJE0003130)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This IL25 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL25
Gene ID: 64806
Uniprot ID: IL25_HUMAN
Specificity: This kit recognizes Human IL-25 in samples. No significant cross-reactivity or interference between Human IL-25 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13.
Protein Name Interleukin-25
Il-25
Interleukin-17e
Il-17e
Database Links Reactome: R-HSA-448424
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-25 ELISA kit
Il-25 ELISA kit
Interleukin-17e ELISA kit
Il-17e ELISA kit
IL25 ELISA kit
IL17E ELISA kit
UNQ3120 ELISA kit
PRO10272 ELISA kit
Specificity This kit recognizes Human IL-25 in samples. No significant cross-reactivity or interference between Human IL-25 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-95 90
EDTA plasma (n=8) 91-107 98
Cell culture media (n=8) 90-102 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 106.78 191.80 690.75 111.06 188.28 758.46
Standard deviation 5.93 8.17 28.74 6.33 8.51 39.74
CV (%) 5.55 4.26 4.16 5.70 4.52 5.24
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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