产品详情

  • 产品名称：Human IL2RA (Interleukin-2 receptor subunit alpha) Sandwich ELISA (STJE0002668)

  • 产品型号：STJE0002668
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL2RA (Interleukin-2 receptor subunit alpha) Sandwich ELISA (STJE0002668)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 56.25pg/mL
Detection Limit: 93.75~6000pg/mL
Short Description: This IL2RA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL2RA
Gene ID: 3559
Uniprot ID: IL2RA_HUMAN
Specificity: This kit recognizes Human IL-2R Alpha in samples. No significant cross-reactivity or interference between Human IL-2R Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Interleukin-2 Receptor Subunit Alpha
Il-2 Receptor Subunit Alpha
Il-2-Ra
Il-2r Subunit Alpha
Il2-Ra
Tac Antigen
P55
Cd Antigen Cd25
Database Links Reactome: R-HSA-5673001
Reactome: R-HSA-8877330
Reactome: R-HSA-9020558
Reactome: R-HSA-912526
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Interleukin-2 Receptor Subunit Alpha ELISA kit
Il-2 Receptor Subunit Alpha ELISA kit
Il-2-Ra ELISA kit
Il-2r Subunit Alpha ELISA kit
Il2-Ra ELISA kit
Tac Antigen ELISA kit
P55 ELISA kit
Cd Antigen Cd25 ELISA kit
IL2RA ELISA kit
Specificity This kit recognizes Human IL-2Rα in samples. No significant cross-reactivity or interference between Human IL-2Rα and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-103 94
EDTA plasma (n=8) 84-100 91
Cell culture media (n=8) 91-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 295.49 623.35 2430.39 307.44 595.04 2280.42
Standard deviation 16.75 30.05 122.25 19.83 23.92 85.06
CV (%) 5.67 4.82 5.03 6.45 4.02 3.73
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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