产品详情

  • 产品名称：Human IL4 (Interleukin-4) Sandwich ELISA (STJE0001991)

  • 产品型号：STJE0001991
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL4 (Interleukin-4) Sandwich ELISA (STJE0001991)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This IL4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL4
Gene ID: 3565
Uniprot ID: IL4_HUMAN
Specificity: This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Interleukin-4
Il-4
B-Cell Stimulatory Factor 1
Bsf-1
Binetrakin
Lymphocyte Stimulatory Factor 1
Pitrakinra
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9012546
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-4 ELISA kit
Il-4 ELISA kit
B-Cell Stimulatory Factor 1 ELISA kit
Bsf-1 ELISA kit
Binetrakin ELISA kit
Lymphocyte Stimulatory Factor 1 ELISA kit
Pitrakinra ELISA kit
IL4 ELISA kit
Specificity This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 93-108 98
Cell culture media (n=8) 90-104 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 102.80 203.60 960.70 107.70 191.50 872.50
Standard deviation 6.20 8.60 33.60 6.60 9.60 26.20
CV (%) 6.03 4.22 3.50 6.13 5.01 3.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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