产品详情

  • 产品名称：Human LGALS3 (Galectin-3) Sandwich ELISA (STJE0002996)

  • 产品型号：STJE0002996
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human LGALS3 (Galectin-3) Sandwich ELISA (STJE0002996)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This LGALS3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LGALS3
Gene ID: 3958
Uniprot ID: LEG3_HUMAN
Specificity: This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Protein Name Galectin-3
Gal-3
35 Kda Lectin
Carbohydrate-Binding Protein 35
Cbp 35
Galactose-Specific Lectin 3
Galactoside-Binding Protein
Galbp
Ige-Binding Protein
L-31
Laminin-Binding Protein
Lectin L-29
Mac-2 Antigen
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-8939246
Reactome: R-HSA-8941333
Cellular Localisation Cytoplasm
Nucleus
Secreted
Secreted By A Non-Classical Secretory Pathway And Associates With The Cell Surface
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocations From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative ELISA Names Galectin-3 ELISA kit
Gal-3 ELISA kit
35 Kda Lectin ELISA kit
Carbohydrate-Binding Protein 35 ELISA kit
Cbp 35 ELISA kit
Galactose-Specific Lectin 3 ELISA kit
Galactoside-Binding Protein ELISA kit
Galbp ELISA kit
Ige-Binding Protein ELISA kit
L-31 ELISA kit
Laminin-Binding Protein ELISA kit
Lectin L-29 ELISA kit
Mac-2 Antigen ELISA kit
LGALS3 ELISA kit
MAC2 ELISA kit
Specificity This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 94-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.55 1.03 3.99 0.54 0.96 3.64
Standard deviation 0.04 0.05 0.18 0.03 0.05 0.20
CV (%) 7.27 4.85 4.51 5.56 5.21 5.49
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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