产品详情

  • 产品名称：Human MME (Neprilysin) Sandwich ELISA (STJE0002492)

  • 产品型号：STJE0002492
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MME (Neprilysin) Sandwich ELISA (STJE0002492)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This MME Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MME
Gene ID: 4311
Uniprot ID: NEP_HUMAN
Specificity: This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Protein Name Neprilysin
Atriopeptidase
Common Acute Lymphocytic Leukemia Antigen
Calla
Enkephalinase
Neutral Endopeptidase 24.11
Nep
Neutral Endopeptidase
Skin Fibroblast Elastase
Sfe
Cd Antigen Cd10
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-5578768
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative ELISA Names Neprilysin ELISA kit
Atriopeptidase ELISA kit
Common Acute Lymphocytic Leukemia Antigen ELISA kit
Calla ELISA kit
Enkephalinase ELISA kit
Neutral Endopeptidase 24.11 ELISA kit
Nep ELISA kit
Neutral Endopeptidase ELISA kit
Skin Fibroblast Elastase ELISA kit
Sfe ELISA kit
Cd Antigen Cd10 ELISA kit
MME ELISA kit
EPN ELISA kit
Specificity This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-109 102
EDTA plasma (n=8) 96-112 102
Cell culture media (n=8) 94-110 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.49 1.16 3.64 0.53 1.07 3.70
Standard deviation 0.03 0.06 0.18 0.03 0.05 0.12
CV (%) 6.12 5.17 4.95 5.66 4.67 3.24
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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