产品详情

  • 产品名称：Human MMP12 (Macrophage metalloelastase) CLIA Kit (STJC0000113)

  • 产品型号：STJC0000113
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MMP12 (Macrophage metalloelastase) CLIA Kit (STJC0000113)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This human MMP12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: MMP12
Gene ID: 4321
Uniprot ID: MMP12_HUMAN
Specificity: This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Found in alveolar macrophages but not in peripheral blood monocytes.
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Protein Name Macrophage Metalloelastase
Mme
Macrophage Elastase
Me
Hme
Matrix Metalloproteinase-12
Mmp-12
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Macrophage Metalloelastase CLIA kit
Mme CLIA kit
Macrophage Elastase CLIA kit
Me CLIA kit
Hme CLIA kit
Matrix Metalloproteinase-12 CLIA kit
Mmp-12 CLIA kit
MMP12 CLIA kit
HME CLIA kit
Specificity This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma(n=8) 96-113 103
Cell culture media(n=8) 90-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 263.96 689.48 2461.65 274.56 670.92 2684.98
Standard deviation 28.53 80.88 149.91 33.47 63.20 202.72
CV (%) 10.81 11.73 6.09 12.19 9.42 7.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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