产品详情

  • 产品名称：Human MMP14 (Matrix metalloproteinase-14) Sandwich ELISA (STJE0003726)

  • 产品型号：STJE0003726
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MMP14 (Matrix metalloproteinase-14) Sandwich ELISA (STJE0003726)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This MMP14 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MMP14
Gene ID: 4323
Uniprot ID: MMP14_HUMAN
Specificity: This kit recognizes Human MMP-14 in samples. No significant cross-reactivity or interference between Human MMP-14 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK.
Function Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
Protein Name Matrix Metalloproteinase-14
Mmp-14
Mmp-X1
Membrane-Type Matrix Metalloproteinase 1
Mt-Mmp 1
Mtmmp1
Membrane-Type-1 Matrix Metalloproteinase
Mt1-Mmp
Mt1mmp
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Melanosome
Cytoplasm
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Forms A Complex With Bst2 And Localizes To The Cytoplasm
Alternative ELISA Names Matrix Metalloproteinase-14 ELISA kit
Mmp-14 ELISA kit
Mmp-X1 ELISA kit
Membrane-Type Matrix Metalloproteinase 1 ELISA kit
Mt-Mmp 1 ELISA kit
Mtmmp1 ELISA kit
Membrane-Type-1 Matrix Metalloproteinase ELISA kit
Mt1-Mmp ELISA kit
Mt1mmp ELISA kit
MMP14 ELISA kit
Specificity This kit recognizes Human MMP-14 in samples. No significant cross-reactivity or interference between Human MMP-14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 85-98 92
Cell culture media (n=8) 86-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.15 6.12 17.04 2.36 6.21 18.28
Standard deviation 0.14 0.34 0.78 0.12 0.33 0.67
CV (%) 6.51 5.56 4.58 5.08 5.31 3.67
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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