产品详情

  • 产品名称：Human NAMPT (Nicotinamide phosphoribosyltransferase) CLIA Kit (STJC0000780)

  • 产品型号：STJC0000780
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human NAMPT (Nicotinamide phosphoribosyltransferase) CLIA Kit (STJC0000780)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human NAMPT kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: NAMPT
Gene ID: 10135
Uniprot ID: NAMPT_HUMAN
Specificity: This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Function Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosesynthesis of NAD. It is the rate limiting component in the mammalian NAD biosesynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
Protein Name Nicotinamide Phosphoribosyltransferase
Namprtase
Nampt
Pre-B-Cell Colony-Enhancing Factor 1
Pre-B Cell-Enhancing Factor
Visfatin
Database Links Reactome: R-HSA-1368108
Reactome: R-HSA-197264
Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Secreted
Under Non-Inflammatory Conditions
Visfatin Predominantly Exhibits A Granular Pattern Within The Nucleus
Secreted By Endothelial Cells Upon Il-1beta Stimulation
Abundantly Secreted In Milk
Reaching 100-Fold Higher Concentrations Compared To Maternal Serum
Alternative CLIA Names Nicotinamide Phosphoribosyltransferase CLIA kit
Namprtase CLIA kit
Nampt CLIA kit
Pre-B-Cell Colony-Enhancing Factor 1 CLIA kit
Pre-B Cell-Enhancing Factor CLIA kit
Visfatin CLIA kit
NAMPT CLIA kit
PBEF CLIA kit
PBEF1 CLIA kit
Specificity This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-114 107
EDTA plasma(n=8) 90-102 97
Cell culture media(n=8) 87-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.85 44.62 180.11 23.30 43.30 177.99
Standard deviation 2.80 5.27 20.42 2.15 4.14 15.91
CV (%) 10.83 11.81 11.34 9.23 9.56 8.94
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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