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  • 产品名称：Human NPY (Pro-neuropeptide Y) Competitive ELISA (STJE0003301)

  • 产品型号：STJE0003301
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human NPY (Pro-neuropeptide Y) Competitive ELISA (STJE0003301)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This NPY Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: NPY
Gene ID: 4852
Uniprot ID: NPY_HUMAN
Specificity: This kit recognizes Human NPY in samples. No significant cross-reactivity or interference between Human NPY and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Function NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Protein Name Pro-Neuropeptide Y Cleaved Into - Neuropeptide Y
Neuropeptide Tyrosine
Npy - C-Flanking Peptide Of Npy
Cpon
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Neuronal Dense Core Vesicle
Alternative ELISA Names Pro-Neuropeptide Y Cleaved Into - Neuropeptide Y ELISA kit
Neuropeptide Tyrosine ELISA kit
Npy - C-Flanking Peptide Of Npy ELISA kit
Cpon ELISA kit
NPY ELISA kit
Specificity This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 91
EDTA plasma(n=8) 92-106 100
Cell culture media(n=8) 87-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 99.80 187.00 734.30 100.00 174.50 759.20
Standard deviation 6.60 8.20 25.00 6.20 7.70 29.60
CV (%) 6.61 4.39 3.40 6.20 4.41 3.90
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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