产品详情

  • 产品名称：Human PLVAP (Plasmalemma vesicle-associated protein) CLIA Kit (STJC0000513)

  • 产品型号：STJC0000513
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PLVAP (Plasmalemma vesicle-associated protein) CLIA Kit (STJC0000513)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human PLVAP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PLVAP
Gene ID: 83483
Uniprot ID: PLVAP_HUMAN
Specificity: This kit recognizes Human PLVAP in samples. No significant cross-reactivity or interference between Human PLVAP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
Function Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development.
Protein Name Plasmalemma Vesicle-Associated Protein
Fenestrated Endothelial-Linked Structure Protein
Plasmalemma Vesicle Protein 1
Pv-1
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Membrane
Caveola
Cytoplasm
Perinuclear Region
Membrane-Associated Protein Of Caveolae
Found In Fenestral And Stomatal Diaphragms In Fenestrated Endothelia And Transendothelial Channels
Also Colocalized With Cav1 In Perinuclear Region
Alternative CLIA Names Plasmalemma Vesicle-Associated Protein CLIA kit
Fenestrated Endothelial-Linked Structure Protein CLIA kit
Plasmalemma Vesicle Protein 1 CLIA kit
Pv-1 CLIA kit
PLVAP CLIA kit
FELS CLIA kit
PV1 CLIA kit
Specificity This kit recognizes Human PLVAP in samples. No significant cross-reactivity or interference between Human PLVAP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma(n=8) 92-106 100
Cell culture media(n=8) 85-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 98.60 177.55 877.12 106.17 160.25 887.11
Standard deviation 8.60 20.13 75.26 13.69 13.93 67.33
CV (%) 8.72 11.34 8.58 12.89 8.69 7.59
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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