产品详情

  • 产品名称：Human Pro-ADM (Proadrenomedullin) Sandwich ELISA (STJE0003874)

  • 产品型号：STJE0003874
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human Pro-ADM (Proadrenomedullin) Sandwich ELISA (STJE0003874)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47pmol/mL
Detection Limit: 0.78~50pmol/mL
Short Description: This Pro-ADM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ADM
Gene ID: 133
Uniprot ID: ADML_HUMAN
Specificity: This kit recognizes Human Pro-ADM in samples. No significant cross-reactivity or interference between Human Pro-ADM and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
Protein Name Pro-Adrenomedullin Cleaved Into - Adrenomedullin
Am - Proadrenomedullin N-20 Terminal Peptide
Proam N-Terminal 20 Peptide
Pamp
Proam-N20
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-419812
Cellular Localisation Secreted
Alternative ELISA Names Pro-Adrenomedullin Cleaved Into - Adrenomedullin ELISA kit
Am - Proadrenomedullin N-20 Terminal Peptide ELISA kit
Proam N-Terminal 20 Peptide ELISA kit
Pamp ELISA kit
Proam-N20 ELISA kit
ADM ELISA kit
AM ELISA kit
Specificity This kit recognizes Human Pro-ADM in samples. No significant cross-reactivity or interference between Human Pro-ADM and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 99
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 94-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pmol/mL) 2.43 7.94 19.47 2.37 7.20 18.19
Standard deviation 0.14 0.39 0.81 0.14 0.39 0.65
CV (%) 5.76 4.91 4.16 5.91 5.42 3.57
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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