产品详情
简单介绍:
Human PROK1 (Prokineticin-1) CLIA Kit (STJC0000056)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 4.69pg/mL |
Detection Limit: | 7.81~500pg/mL |
Short Description: | This human PROK1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | PROK1 |
Gene ID: | 84432 |
Uniprot ID: | PROK1_HUMAN |
Specificity: | This kit recognizes Human EG-VEGF in samples. No significant cross-reactivity or interference between Human EG-VEGF and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Tissue Specificity | Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. |
Function | Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. |
Protein Name | Prokineticin-1 Endocrine-Gland-Derived Vascular Endothelial Growth Factor Eg-Vegf Mambakine |
Database Links | Reactome: R-HSA-375276 Reactome: R-HSA-416476 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Prokineticin-1 CLIA kit Endocrine-Gland-Derived Vascular Endothelial Growth Factor CLIA kit Eg-Vegf CLIA kit Mambakine CLIA kit PROK1 CLIA kit UNQ600 CLIA kit PRO1186 CLIA kit |
Specificity | This kit recognizes Human EG-VEGF in samples. No significant cross-reactivity or interference between Human EG-VEGF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 87-98 | 92 |
EDTA plasma(n=8) | 100-116 | 108 |
Cell culture media(n=8) | 94-111 | 102 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 25.41 | 58.22 | 174.39 | 27.60 | 55.19 | 160.82 |
Standard deviation | 2.13 | 5.75 | 16.20 | 2.92 | 6.53 | 14.52 |
CV (%) | 8.38 | 9.88 | 9.29 | 10.58 | 11.83 | 9.03 |
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