产品详情

  • 产品名称：Human S100A12 (Protein S100-A12) Sandwich ELISA (STJE0002860)

  • 产品型号：STJE0002860
  • 产品厂商：ST john's laboratory
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Human S100A12 (Protein S100-A12) Sandwich ELISA (STJE0002860)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This S100A12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: S100A12
Gene ID: 6283
Uniprot ID: S10AC_HUMAN
Specificity: This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases.MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.
Protein Name Protein S100-A12
Cgrp
Calcium-Binding Protein In Amniotic Fluid 1
Caaf1
Calgranulin-C
Cagc
Extracellular Newly Identified Rage-Binding Protein
En-Rage
Migration Inhibitory Factor-Related Protein 6
Mrp-6
P6
Neutrophil S100 Protein
S100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin
Database Links Reactome: R-HSA-445989
Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-933542
Cellular Localisation Secreted
Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Predominantly Localized In The Cytoplasm
Upon Elevation Of The Intracellular Calcium Level
Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane
Upon Neutrophil Activation Is Secreted Via A Microtubule-Mediated
Alternative Pathway
Alternative ELISA Names Protein S100-A12 ELISA kit
Cgrp ELISA kit
Calcium-Binding Protein In Amniotic Fluid 1 ELISA kit
Caaf1 ELISA kit
Calgranulin-C ELISA kit
Cagc ELISA kit
Extracellular Newly Identified Rage-Binding Protein ELISA kit
En-Rage ELISA kit
Migration Inhibitory Factor-Related Protein 6 ELISA kit
Mrp-6 ELISA kit
P6 ELISA kit
Neutrophil S100 Protein ELISA kit
S100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin ELISA kit
S100A12 ELISA kit
Specificity This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 98
EDTA plasma (n=8) 91-107 98
Cell culture media (n=8) 89-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.49 1.08 4.12 0.48 1.06 3.86
Standard deviation 0.03 0.05 0.14 0.03 0.05 0.15
CV (%) 6.12 4.63 3.40 6.25 4.72 3.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
姓名：
电话：
您的需求：
 
网站地图