产品详情

  • 产品名称：Human MMP1 (Matrix Metalloproteinase 1) Sandwich ELISA (STJE0004085)

  • 产品型号：STJE0004085
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MMP1 (Matrix Metalloproteinase 1) Sandwich ELISA (STJE0004085)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This MMP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MMP1
Gene ID: 4312
Uniprot ID: MMP1_HUMAN
Specificity: This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Protein Name Interstitial Collagenase
Fibroblast Collagenase
Matrix Metalloproteinase-1
Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-210991
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Interstitial Collagenase ELISA kit
Fibroblast Collagenase ELISA kit
Matrix Metalloproteinase-1 ELISA kit
Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase ELISA kit
MMP1 ELISA kit
CLG ELISA kit
Specificity This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 82-98 90
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 98-108 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 85.33 246.42 880.11 82.77 242.81 921.97
Standard deviation 4.95 11.58 36.08 4.59 11.07 47.02
CV (%) 5.80 4.70 4.10 5.55 4.56 5.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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