产品详情

  • 产品名称：Human POSTN/OSF-2 (Periosestin) Sandwich ELISA (STJE0019688)

  • 产品型号：STJE0019688
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human POSTN/OSF-2 (Periosestin) Sandwich ELISA (STJE0019688)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16-10ng/mL
Short Description: This POSTN/OSF-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: POSTN
Gene ID: 10631
Uniprot ID: POSTN_HUMAN
Specificity: This kit recognizes Human POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Human POSTN/OSF-2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.
Function Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
Protein Name Periosestin
Pn
Osteoblast-Specific Factor 2
Osf-2
Cellular Localisation Golgi Apparatus
Secreted
Extracellular Space
Extracellular Matrix
Colocalizes With Bmp1 In The Golgi
Alternative ELISA Names Periosestin ELISA kit
Pn ELISA kit
Osteoblast-Specific Factor 2 ELISA kit
Osf-2 ELISA kit
POSTN ELISA kit
OSF2 ELISA kit
Specificity This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma (n=8) 91-102 96
Cell culture media (n=8) 96-108 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 0.98 4.20 0.47 0.95 4.35
Standard deviation 0.03 0.04 0.13 0.03 0.05 0.15
CV (%) 6.30 4.00 3.09 5.38 5.43 3.34
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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