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  • 产品名称：Human SFRP2 (Secreted frizzled-related protein 2) Sandwich ELISA (STJE0002874)

  • 产品型号：STJE0002874
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SFRP2 (Secreted frizzled-related protein 2) Sandwich ELISA (STJE0002874)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.75ng/mL
Detection Limit: 1.25~80ng/mL
Short Description: This SFRP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SFRP2
Gene ID: 6423
Uniprot ID: SFRP2_HUMAN
Specificity: This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Protein Name Secreted Frizzled-Related Protein 2
Frp-2
Sfrp-2
Secreted Apoptosis-Related Protein 1
Sarp-1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Alternative ELISA Names Secreted Frizzled-Related Protein 2 ELISA kit
Frp-2 ELISA kit
Sfrp-2 ELISA kit
Secreted Apoptosis-Related Protein 1 ELISA kit
Sarp-1 ELISA kit
SFRP2 ELISA kit
FRP2 ELISA kit
SARP1 ELISA kit
FKSG12 ELISA kit
UNQ361 ELISA kit
PRO697 ELISA kit
Specificity This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-110 100
EDTA plasma (n=8) 91-105 97
Cell culture media (n=8) 85-97 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 3.77 7.03 29.83 4.00 7.14 30.06
Standard deviation 0.19 0.31 1.27 0.27 0.40 1.05
CV (%) 5.04 4.41 4.26 6.75 5.60 3.49
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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