产品详情

  • 产品名称：Human TFPI (Tissue factor pathway inhibitor) CLIA Kit (STJC0000130)

  • 产品型号：STJC0000130
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TFPI (Tissue factor pathway inhibitor) CLIA Kit (STJC0000130)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human TFPI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TFPI
Gene ID: 7035
Uniprot ID: TFPI1_HUMAN
Specificity: This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Mostly in endothelial cells.
Post Translational Modifications O-glycosylated.
Function Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Protein Name Tissue Factor Pathway Inhibitor
Tfpi
Extrinsic Pathway Inhibitor
Epi
Lipoprotein-Associated Coagulation Inhibitor
Laci
Database Links Reactome: R-HSA-140834
Cellular Localisation Isoform Alpha: Secreted
Isoform Beta: Microsome Membrane
Lipid-Anchor
Gpi-Anchor
Alternative CLIA Names Tissue Factor Pathway Inhibitor CLIA kit
Tfpi CLIA kit
Extrinsic Pathway Inhibitor CLIA kit
Epi CLIA kit
Lipoprotein-Associated Coagulation Inhibitor CLIA kit
Laci CLIA kit
TFPI CLIA kit
LACI CLIA kit
TFPI1 CLIA kit
Specificity This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma(n=8) 95-109 103
Cell culture media(n=8) 97-114 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.02 278.52 872.74 100.06 252.51 820.70
Standard deviation 12.23 29.86 69.91 9.28 24.27 89.54
CV (%) 11.87 10.72 8.01 9.27 9.61 10.91
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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