产品详情

  • 产品名称：Mouse S100a8 (Protein S100-A8) Sandwich ELISA (STJE0005364)

  • 产品型号：STJE0005364
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse S100a8 (Protein S100-A8) Sandwich ELISA (STJE0005364)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This S100a8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: S100a8
Gene ID: 20201
Uniprot ID: S10A8_MOUSE
Specificity: This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Protein S100-A8
Calgranulin-A
Chemotactic Cytokine Cp-10
Leukocyte L1 Complex Light Chain
Migration Inhibitory Factor-Related Protein 8
Mrp-8
P8
Pro-Inflammatory S100 Cytokine
S100 Calcium-Binding Protein A8
Database Links Reactome: R-MMU-5668599
Reactome: -MMU-5686938
Reactome: -MMU-6798695
Reactome: -MMU-6799990
Cellular Localisation Secreted
Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Predominantly Localized In The Cytoplasm
Upon Elevation Of The Intracellular Calcium Level
Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane
Upon Neutrophil Activation Or Endothelial Adhesion Of Monocytes
Is Secreted Via A Microtubule-Mediated
Alternative Pathway
Alternative ELISA Names Protein S100-A8 ELISA kit
Calgranulin-A ELISA kit
Chemotactic Cytokine Cp-10 ELISA kit
Leukocyte L1 Complex Light Chain ELISA kit
Migration Inhibitory Factor-Related Protein 8 ELISA kit
Mrp-8 ELISA kit
P8 ELISA kit
Pro-Inflammatory S100 Cytokine ELISA kit
S100 Calcium-Binding Protein A8 ELISA kit
S100a8 ELISA kit
Caga ELISA kit
Mrp8 ELISA kit
Specificity This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 93
EDTA plasma (n=8) 83-95 90
Cell culture media (n=8) 90-103 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 188.64 414.53 1934.43 195.77 446.65 1946.95
Standard deviation 11.11 20.60 92.08 11.39 20.64 78.07
CV (%) 5.89 4.97 4.76 5.82 4.62 4.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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