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  • 产品名称：Mouse SELP (P-Selectin) Sandwich ELISA (STJE0019743)

  • 产品型号：STJE0019743
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse SELP (P-Selectin) Sandwich ELISA (STJE0019743)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16-10pg/mL
Short Description: This SELP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Selp
Gene ID: 20344
Uniprot ID: LYAM3_MOUSE
Specificity: This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
Protein Name P-Selectin
Cd62 Antigen-Like Family Member P
Granule Membrane Protein 140
Gmp-140
Leukocyte-Endothelial Cell Adhesion Molecule 3
Lecam3
Platelet Activation Dependent Granule-External Membrane Protein
Padgem
Cd Antigen Cd62p
Database Links Reactome: R-MMU-114608
Reactome: -MMU-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names P-Selectin ELISA kit
Cd62 Antigen-Like Family Member P ELISA kit
Granule Membrane Protein 140 ELISA kit
Gmp-140 ELISA kit
Leukocyte-Endothelial Cell Adhesion Molecule 3 ELISA kit
Lecam3 ELISA kit
Platelet Activation Dependent Granule-External Membrane Protein ELISA kit
Padgem ELISA kit
Cd Antigen Cd62p ELISA kit
Selp ELISA kit
Grmp ELISA kit
Specificity This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 96
EDTA plasma (n=8) 88-100 95
Cell culture media (n=8) 92-105 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.54 1.59 4.86 0.52 1.69 4.48
Standard deviation 0.03 0.08 0.19 0.03 0.09 0.22
CV (%) 5.93 5.33 3.87 5.37 5.08 4.84
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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