产品详情

  • 产品名称：Mouse Tgfb2 (Transforming growth factor beta-2 proprotein) Sandwich ELISA (STJE0005071)

  • 产品型号：STJE0005071
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Tgfb2 (Transforming growth factor beta-2 proprotein) Sandwich ELISA (STJE0005071)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Tgfb2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Tgfb2
Gene ID: 21808
Uniprot ID: TGFB2_MOUSE
Specificity: This kit recognizes Mouse TGF-Beta 2 in samples. No significant cross-reactivity or interference between Mouse TGF-Beta 2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
Protein Name Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated Peptide
Lap - Transforming Growth Factor Beta-2
Tgf-Beta-2
Database Links Reactome: R-MMU-114608
Reactome: -MMU-2129379
Reactome: -MMU-2173789
Cellular Localisation Latency-Associated Peptide: Secreted
Extracellular Space
Extracellular Matrix
Transforming Growth Factor Beta-2: Secreted
Alternative ELISA Names Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated Peptide ELISA kit
Lap - Transforming Growth Factor Beta-2 ELISA kit
Tgf-Beta-2 ELISA kit
Tgfb2 ELISA kit
Specificity This kit recognizes Mouse TGF-β2 in samples. No significant cross-reactivity or interference between Mouse TGF-β2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-106 99
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 88-99 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.37 84.50 499.92 49.73 76.67 544.03
Standard deviation 2.76 4.53 21.85 2.63 3.98 20.13
CV (%) 5.37 5.36 4.37 5.29 5.19 3.70
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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