產品詳情
簡單介紹:
Mouse CRT (Calreticulin) CLIA Kit (STJC0001124)
詳情介紹:
Applications: | CLIA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 18.75pg/mL |
Detection Limit: | 31.25~2000pg/mL |
Short Description: | This mouse CRT kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | Calr |
Gene ID: | 12317 |
Uniprot ID: | CALR_MOUSE |
Specificity: | This kit recognizes Mouse CRT in samples. No significant cross-reactivity or interference between Mouse CRT and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy. |
Protein Name | Calreticulin Crp55 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp |
Database Links | Reactome: R-MMU-1236974 Reactome: -MMU-3000480 Reactome: -MMU-901042 Reactome: -MMU-983170 |
Cellular Localisation | Endoplasmic Reticulum Lumen Cytoplasm Cytosol Cytolytic Granule Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation |
Alternative CLIA Names | Calreticulin CLIA kit Crp55 CLIA kit Calregulin CLIA kit Endoplasmic Reticulum Resident Protein 60 CLIA kit Erp60 CLIA kit Hacbp CLIA kit Calr CLIA kit |
Specificity | This kit recognizes Mouse CRT in samples. No significant cross-reactivity or interference between Mouse CRT and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 96-108 | 102 |
EDTA plasma(n=8) | 98-113 | 105 |
Cell culture media(n=8) | 99-114 | 107 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 104.86 | 222.32 | 703.65 | 101.22 | 212.10 | 706.40 |
Standard deviation | 13.21 | 25.19 | 72.76 | 13.10 | 22.16 | 72.76 |
CV (%) | 12.60 | 11.33 | 10.34 | 12.94 | 10.45 | 10.30 |
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