產品詳情
簡單介紹：
Mouse EGFR (Epidermal Growth Factor Receptor) Sandwich ELISA (STJE0019704)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 3.75pg/mL |
| Detection Limit: | 6.25-400pg/mL |
| Short Description: | This EGFR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Egfr |
| Gene ID: | 13649 |
| Uniprot ID: | EGFR_MOUSE |
| Specificity: | This kit recognizes Mouse EGFR in samples. No significant cross-reactivity or interference between Mouse EGFR and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration. Plays a role in enhancing learning and memory performance. Plays a role in mammalian pain signaling (long-lasting hypersensitivity). |
| Protein Name | Epidermal Growth Factor Receptor |
| Database Links | Reactome: R-MMU-1227986 Reactome: -MMU-1236394 Reactome: -MMU-1250196 Reactome: -MMU-1257604 Reactome: -MMU-177929 Reactome: -MMU-179812 Reactome: -MMU-180292 Reactome: -MMU-180336 Reactome: -MMU-182971 Reactome: -MMU-1963642 Reactome: -MMU-212718 Reactome: -MMU-2179392 Reactome: -MMU-445144 Reactome: -MMU-5673001 Reactome: -MMU-6785631 Reactome: -MMU-6811558 Reactome: -MMU-8847993 Reactome: -MMU-8856825 Reactome: -MMU-8856828 Reactome: -MMU-8857538 Reactome: -MMU-8863795 Reactome: -MMU-9009391 Reactome: -MMU-9013507 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Membrane Golgi Apparatus Membrane Nucleus Membrane Endosome Endosome Membrane Nucleus In Response To Egf Translocated From The Cell Membrane To The Nucleus Via Golgi And Er Endocytosed Upon Activation By Ligand Colocalized With Gper1 In The Nucleus Of Estrogen Agonist-Induced Cancer-Associated Fibroblasts (Caf) |
| Alternative ELISA Names | Epidermal Growth Factor Receptor ELISA kit Egfr ELISA kit |
| Specificity | This kit recognizes Mouse EGFR in samples. No significant cross-reactivity or interference between Mouse EGFR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 90-102 | 96 |
| EDTA plasma (n=8) | 96-107 | 101 |
| Cell culture media (n=8) | 86-101 | 93 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 19.12 | 47.06 | 146.64 | 17.69 | 42.37 | 151.93 |
| Standard deviation | 1.08 | 2.43 | 4.78 | 0.89 | 1.86 | 6.41 |
| CV (%) | 5.66 | 5.16 | 3.26 | 5.02 | 4.40 | 4.22 |
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