產品詳情
簡單介紹:
Mouse FETUA (Fetuin A) Sandwich ELISA (STJE0019982)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.03 Mu g/mL |
Detection Limit: | 0.08-5 ug/mL |
Short Description: | This FETUA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Ahsg |
Gene ID: | 11625 |
Uniprot ID: | FETUA_MOUSE |
Specificity: | This kit recognizes Mouse FETUA in samples. No significant cross-reactivity or interference between Mouse FETUA and analogues was observed. |
Sample Type: | serum, plasma and other biological fluids |
Tissue Specificity | Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development. |
Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. |
Function | Probably involved in differentiation. |
Protein Name | Alpha-2-Hs-Glycoprotein Countertrypin Fetuin-A |
Database Links | Reactome: R-MMU-114608 Reactome: -MMU-381426 Reactome: -MMU-6798695 Reactome: -MMU-8957275 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Alpha-2-Hs-Glycoprotein ELISA kit Countertrypin ELISA kit Fetuin-A ELISA kit Ahsg ELISA kit Fetua ELISA kit |
Specificity | This kit recognizes Mouse FETUA in samples. No significant cross-reactivity or interference between Mouse FETUA and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 89-103 | 94 |
EDTA plasma (n=8) | 93-106 | 98 |
Cell culture media (n=8) | 85-96 | 90 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (μg/mL) | 0.25 | 0.79 | 1.99 | 0.26 | 0.72 | 2.09 |
Standard deviation | 0.02 | 0.05 | 0.07 | 0.01 | 0.03 | 0.11 |
CV (%) | 6.06 | 5.81 | 3.32 | 5.22 | 4.83 | 5.22 |
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