| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
3.75ng/mL |
| Detection Limit: |
6.25~400ng/mL |
| Short Description: |
This IgG1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Specificity: |
This kit recognizes Mouse IgG1 in samples. No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Specificity | This kit recognizes Mouse IgG1 in samples. No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
Item Specifications Storage
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
Sample Type Range (%) Average Recovery (%)
| Serum(n=8) | 94-108 | 101 |
| EDTA plasma (n=8) | 85-99 | 93 |
| Cell culture media (n=8) | 99-110 | 103 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 16.75 | 46.08 | 163.26 | 18.94 | 44.05 | 194.01 |
| Standard deviation | 0.95 | 2.16 | 8.39 | 1.13 | 2.04 | 10.32 |
| CV (%) | 5.67 | 4.69 | 5.14 | 5.97 | 4.63 | 5.32 |
12 months for
antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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