產品詳情
簡單介紹：
Mouse Il33 (Interleukin-33) Sandwich ELISA (STJE0005259)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This Il33 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Il33 |
| Gene ID: | 77125 |
| Uniprot ID: | IL33_MOUSE |
| Specificity: | This kit recognizes Mouse IL-33 in samples. No significant cross-reactivity or interference between Mouse IL-33 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation. |
| Protein Name | Interleukin-33 Il-33 Cleaved Into - Interleukin-33(102-266 - Interleukin-33(109-266 |
| Database Links | Reactome: R-MMU-1257604 Reactome: -MMU-5689880 Reactome: -MMU-6811558 Reactome: -MMU-9014843 |
| Cellular Localisation | Nucleus Chromosome Cytoplasm Cytoplasmic Vesicle Secretory Vesicle Secreted Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore Following Cleavage By Cela1 Associates With Heterochromatin And Mitotic Chromosomes The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 It Results In Protein Translocations From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion (Microbial Infection) Upon Infection With H Polygyrus Tethered To The Nucleus By The H Polygyrus Parasitic Protein Ari |
| Alternative ELISA Names | Interleukin-33 ELISA kit Il-33 Cleaved Into - Interleukin-33(102-266 - Interleukin-33(109-266 ELISA kit Il33 ELISA kit |
| Specificity | This kit recognizes Mouse IL-33 in samples. No significant cross-reactivity or interference between Mouse IL-33 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 95-105 | 100 |
| EDTA plasma (n=8) | 86-98 | 93 |
| Cell culture media (n=8) | 86-99 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.76 | 132.49 | 433.12 | 46.03 | 123.63 | 442.74 |
| Standard deviation | 2.40 | 5.59 | 14.16 | 2.41 | 5.92 | 20.45 |
| CV (%) | 5.03 | 4.22 | 3.27 | 5.24 | 4.79 | 4.62 |
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