產品詳情
簡單介紹：
Mouse LBP (Lipopolysaccharide Binding Protein) Sandwich ELISA (STJE0019914)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 40.21 pg/mL |
| Detection Limit: | 125-8000 pg/mL |
| Short Description: | This LBP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Lbp |
| Gene ID: | 16803 |
| Uniprot ID: | LBP_MOUSE |
| Specificity: | This kit recognizes Mouse LBP in samples. No significant cross-reactivity or interference between Mouse LBP and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Function | Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide. |
| Protein Name | Lipopolysaccharide-Binding Protein Lbp |
| Database Links | Reactome: R-MMU-166016 Reactome: -MMU-166020 Reactome: -MMU-5686938 |
| Cellular Localisation | Secreted Cytoplasmic Granule Membrane Membrane-Associated In Polymorphonuclear Leukocytes (Pmn) Granules |
| Alternative ELISA Names | Lipopolysaccharide-Binding Protein ELISA kit Lbp ELISA kit Lbp ELISA kit |
| Specificity | This kit recognizes Mouse LBP in samples. No significant cross-reactivity or interference between Mouse LBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 92-102 | 97 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 91-102 | 96 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 430.43 | 1137.55 | 3578.56 | 417.17 | 1084.10 | 3563.50 |
| Standard deviation | 24.19 | 57.56 | 154.59 | 27.99 | 49.00 | 193.14 |
| CV (%) | 5.62 | 5.06 | 4.32 | 6.71 | 4.52 | 5.42 |
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