產品詳情
簡單介紹:
Mouse LIPD (Lipase, Lipoprotein) Sandwich ELISA Kit Ready-To-Use (STJE0016740)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Sensitivity: | 24pg/mL |
Detection Limit: | 78.1-5000pg/mL |
Short Description: | This LIPD Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
Assay Time: | 3 hrs |
Gene Symbol: | Lpl |
Gene ID: | 16956 |
Uniprot ID: | LIPL_MOUSE |
Immunogen Region: | Ready-To-Use |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Tissue Specificity | Detected in white and brown adipose tissue and heart muscle, especially at the lumenal surface of capillaries. Detected on capillary endothelium in the lactating mammary gland. Detected in blood plasma (at protein level). Expressed in liver, epididymal fat, heart, psoas muscle, lactating mammary gland, adrenal, lung, and ovary. Highest levels in heart and adrenal gland. |
Post Translational Modifications | Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. N-glycosylated. |
Function | Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. |
Protein Name | Lipoprotein Lipase Lpl Phospholipase A1 |
Database Links | Reactome: R-MMU-8963889 Reactome: -MMU-8963901 Reactome: -MMU-975634 |
Cellular Localisation | Cell Membrane Peripheral Membrane Protein Extracellular Side Secreted Extracellular Space Extracellular Matrix Newly Synthesized Lpl Binds To Cell Surface Heparan Proteoglycans And Is Then Released By Heparanase Subsequently It Becomes Attached To Heparan Proteoglycan On Endothelial Cells Locates To The Plasma Membrane Of Microvilli Of Hepatocytes With Triglyceride-Rich Lipoproteins (Trl) Some Of The Bound Lpl Is Then Internalized And Located Inside Non-Coated Endocytic Vesicles |
Alternative ELISA Names | Lipoprotein Lipase ELISA kit Lpl ELISA kit Phospholipase A1 ELISA kit Lpl ELISA kit |
output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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