產品詳情
簡單介紹:
Mouse PGF (Placental Growth Factor) Sandwich ELISA (STJE0019915)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 1.25 pg/mL |
Detection Limit: | 3.91-250 pg/mL |
Short Description: | This PGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Pgf |
Gene ID: | 18654 |
Uniprot ID: | PLGF_MOUSE |
Specificity: | This kit recognizes Mouse PGF in samples. No significant cross-reactivity or interference between Mouse PGF and analogues was observed. |
Sample Type: | serum, plasma and other biological fluids |
Function | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth. |
Protein Name | Placenta Growth Factor Plgf |
Database Links | Reactome: R-MMU-194313 Reactome: -MMU-195399 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Placenta Growth Factor ELISA kit Plgf ELISA kit Pgf ELISA kit Plgf ELISA kit |
Specificity | This kit recognizes Mouse PGF in samples. No significant cross-reactivity or interference between Mouse PGF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 95-106 | 101 |
EDTA plasma (n=8) | 92-103 | 98 |
Cell culture media (n=8) | 87-101 | 95 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 12.01 | 39.43 | 114.41 | 12.24 | 42.08 | 120.29 |
Standard deviation | 0.63 | 1.69 | 4.46 | 0.67 | 2.05 | 3.83 |
CV (%) | 5.21 | 4.28 | 3.90 | 5.51 | 4.86 | 3.18 |
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