產品詳情
簡單介紹：
Mouse Sdc1 (Syndecan-1) Sandwich ELISA (STJE0005223)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This Sdc1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Sdc1 |
| Gene ID: | 20969 |
| Uniprot ID: | SDC1_MOUSE |
| Specificity: | This kit recognizes Mouse SDC1 in samples. No significant cross-reactivity or interference between Mouse SDC1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway. |
| Protein Name | Syndecan-1 Synd1 Cd Antigen Cd138 |
| Database Links | Reactome: R-MMU-1971475 Reactome: -MMU-2022928 Reactome: -MMU-2024096 Reactome: -MMU-202733 Reactome: -MMU-3000170 Reactome: -MMU-449836 Reactome: -MMU-975634 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Secreted Extracellular Exosome Shedding Of The Ectodomain Produces A Soluble Form |
| Alternative ELISA Names | Syndecan-1 ELISA kit Synd1 ELISA kit Cd Antigen Cd138 ELISA kit Sdc1 ELISA kit Synd-1 ELISA kit Synd1 ELISA kit |
| Specificity | This kit recognizes Mouse SDC1 in samples. No significant cross-reactivity or interference between Mouse SDC1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 89-100 | 94 |
| EDTA plasma (n=8) | 92-106 | 100 |
| Cell culture media (n=8) | 90-106 | 97 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.00 | 4.46 | 0.48 | 1.10 | 4.68 |
| Standard deviation | 0.03 | 0.04 | 0.20 | 0.03 | 0.06 | 0.16 |
| CV (%) | 6.52 | 4.00 | 4.48 | 6.25 | 5.45 | 3.42 |
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