產品詳情
簡單介紹：
Mouse Slc6a3 (Sodium-dependent dopamine transporter) Sandwich ELISA (STJE0004514)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This Slc6a3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Slc6a3 |
| Gene ID: | 13162 |
| Uniprot ID: | SC6A3_MOUSE |
| Specificity: | This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling. |
| Protein Name | Sodium-Dependent Dopamine Transporter Da Transporter Dat Solute Carrier Family 6 Member 3 |
| Database Links | Reactome: R-MMU-379401 Reactome: -MMU-442660 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cell Projection Neuron Projection Axon Localizes To Neurite Tips In Neuronal Cells Colocalizes With Septin4 At Axon Terminals Especially At The Varicosities |
| Alternative ELISA Names | Sodium-Dependent Dopamine Transporter ELISA kit Da Transporter ELISA kit Dat ELISA kit Solute Carrier Family 6 Member 3 ELISA kit Slc6a3 ELISA kit Dat ELISA kit Dat1 ELISA kit |
| Specificity | This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma (n=8) | 84-97 | 91 |
| Cell culture media (n=8) | 85-99 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 22.94 | 71.32 | 212.65 | 23.07 | 72.20 | 200.28 |
| Standard deviation | 1.22 | 3.16 | 9.25 | 1.53 | 3.42 | 10.59 |
| CV (%) | 5.32 | 4.43 | 4.35 | 6.63 | 4.74 | 5.29 |
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