產品詳情
簡單介紹：
Mouse TNF-Alpha (Tumor Necrosis Factor Alpha) Sandwich ELISA (STJE0019966)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 2.76 pg/mL |
| Detection Limit: | 4.69-300 pg/mL |
| Short Description: | This TNF-Alpha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Tnf |
| Gene ID: | 21926 |
| Uniprot ID: | TNFA_MOUSE |
| Specificity: | This kit recognizes Mouse TNF-Alpha in samples. No significant cross-reactivity or interference between Mouse TNF-Alpha and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Post Translational Modifications | The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated.glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor, soluble form: The soluble form is demyristoylated by SIRT6, promoting its secretion. |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Protein Name | Tumor Necrosis Factor Cachectin Tnf-Alpha Tumor Necrosis Factor Ligand Superfamily Member 2 Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form N-Terminal Fragment Ntf - Intracellular Domain 1 Icd1 - Intracellular Domain 2 Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form |
| Database Links | Reactome: R-MMU-5357786 Reactome: -MMU-5357905 Reactome: -MMU-5357956 Reactome: -MMU-5626978 Reactome: -MMU-5668541 Reactome: -MMU-75893 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Membrane Form: Membrane Soluble Form: Secreted C-Domain 1: Secreted C-Domain 2: Secreted |
| Alternative ELISA Names | Tumor Necrosis Factor ELISA kit Cachectin ELISA kit Tnf-Alpha ELISA kit Tumor Necrosis Factor Ligand Superfamily Member 2 ELISA kit Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form ELISA kit N-Terminal Fragment ELISA kit Ntf - Intracellular Domain 1 ELISA kit Icd1 - Intracellular Domain 2 ELISA kit Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form ELISA kit Tnf ELISA kit Tnfa ELISA kit Tnfsf2 ELISA kit |
| Specificity | This kit recognizes Mouse TNF-α in samples. No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 87-100 | 92 |
| EDTA plasma (n=8) | 91-109 | 99 |
| Cell culture media (n=8) | 94-110 | 100 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 15.17 | 25.68 | 115.41 | 15.93 | 24.66 | 112.05 |
| Standard deviation | 0.91 | 1.09 | 3.59 | 0.93 | 1.25 | 3.56 |
| CV (%) | 6.01 | 4.23 | 3.11 | 5.83 | 5.05 | 3.18 |
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