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Pregnenolone (Pregnenolone) Competitive ELISA (STJE0006614) STJE0006614|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司
產品詳情
  • 產品名稱:Pregnenolone (Pregnenolone) Competitive ELISA (STJE0006614)

  • 產品型號:STJE0006614
  • 產品廠商:ST john's laboratory
  • 產品文檔:
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簡單介紹:
Pregnenolone (Pregnenolone) Competitive ELISA (STJE0006614)
詳情介紹:
Applications: ELISA
Reactivity: All
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This Pregnenolone Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in all cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Specificity: This kit recognizes Pregnenolone in samples. No significant cross-reactivity or interference between Pregnenolone and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Background This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in the sample or standard competes with a fixed amount of Pregnenolone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pregnenolone in the samples is then determined by comparing the OD of the samples to the standard curve.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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