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Mouse PAPPA (Pappalysin-1) CLIA Kit (STJC0001066) STJC0001066|產品目錄|代理商|上海91麻豆福利视频网生物科技有限公司
產品詳情

  • 產品名稱：Mouse PAPPA (Pappalysin-1) CLIA Kit (STJC0001066)

  • 產品型號：STJC0001066
  • 產品廠商：ST john's laboratory
  • 產品文檔：
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簡單介紹：
Mouse PAPPA (Pappalysin-1) CLIA Kit (STJC0001066)
詳情介紹：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This mouse PAPPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Pappa
Gene ID: 18491
Uniprot ID: PAPP1_MOUSE
Specificity: This kit recognizes Mouse PAPPA in samples. No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in kidney, spleen, brain, ovary, breast, skin, prostate, uterus, and placenta.
Function Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.
Protein Name Pappalysin-1
Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease
Igf-Dependent Igfbp-4 Protease
Igfbp-4ase
Pregnancy-Associated Plasma Protein A
Papp-A
Database Links Reactome: R-MMU-381426
Cellular Localisation Secreted
Alternative CLIA Names Pappalysin-1 CLIA kit
Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease CLIA kit
Igf-Dependent Igfbp-4 Protease CLIA kit
Igfbp-4ase CLIA kit
Pregnancy-Associated Plasma Protein A CLIA kit
Papp-A CLIA kit
Pappa CLIA kit
Specificity This kit recognizes Mouse PAPPA in samples. No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-114 105
EDTA plasma(n=8) 95-111 101
Cell culture media(n=8) 96-110 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 96.77 254.44 747.73 97.92 252.88 736.47
Standard deviation 8.45 23.31 85.47 9.59 27.34 57.74
CV (%) 8.73 9.16 11.43 9.79 10.81 7.84
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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